The disease model
Depending on the therapeutic target evaluated, the client may choose a disease model based on a collection of humanized transgenic mice or a wild type animals.
The study design
- Generation of experimental animals & group formation
- Control groups
- Administration route:IV, IP, SC, IM, PO, IR, etc
- In vivo monitoring: measurements, clinical scoring (weight, temperature), general health status
- Biological sample collection: blood, plasma, serum, urine
The assays
- ELISA
- Molecular & cellular assays
- FACS analysis
- Thick organ culture
- Multiplex analysis (with external collaboration)
The histology
- Gross necropsy
- Tissue collection
- Paraffin embedding
- Histopathology slide preparation & staining
- Histopathological evaluation
- Immunohistochemistry
Pharmacogenomics analysis
- RNA preparation
- Gene expression profiling
- Functional analysis
- MOA