In the context of the INFRAFRONTIER 2020 specialized phenotyping program, Biomedcode accepts proposals that will aim in uncovering the role of a gene of interest in inflammatory conditions.
To uncover gene function in pathologic processes we will perform phenotypic analysis of mutant mice based on their response to one acute inflammation and one inflammatory disease model. The user’s research interests, the nature of the mutated gene and the strain of the mutant animal under investigation will dictate the selection of the most appropriate combination of one acute and one chronic model, to provide the most informative customized output for the user.
Acute Models of Inflammation will serve for the evaluation of the involvement of the gene of interest in inflammatory mechanisms through assessment of its effect in proinflammatory cytokine levels and/or inflammatory cell recruitment. Offered acute models include: 1) Lipopolysaccharide (LPS) Induced Cytokine cascade, 2) Zymosan Induced cell recruitment, 3) Thioglucolate Induced cell recruitment
Induced Disease Models will serve for the evaluation of the involvement of the gene interest in a disease setting, assessing its effect in disease development and progression at the clinical and histopathological level. Offered disease models include: 1) Collagen Antibody induced arthritis (CAIA) (*Arthritomab must be provided by the interested party), 2) TNBS-colitis, 3) IMQ-induced psoriasis, 4) MOG-induced Experimental Autoimmune Encephalomyelitis.
3 proposals that fulfill the requirements set in the application form will be processed free of charge.